The MIC of the EtOAc extract against Phytophthora was determined by the modified agar diffusion method ( Espinel-Ingroff et al., 2007). The MIC was determined using the same method used for preliminary screening with three different concentrations of EtOAc extracts, 100, 10 and 1 μg, in 10 μL of acetone–water (1:9, v/v). Seven Phytophthora species were treated with the extracts from eight Trichoderma isolates, which were selected through preliminary screening. Each experiment was repeated two times, with three biological replications per experiment.