The TAG composition can be calculated utilizing fatty
acid methyl ester (FAME) determinations and computer
programs, by applying the 1,3-random 2-random distribution
theory [12]. For this purpose a lipase digestion is
made and resulting lipids are separated by TLC. The
monoacylglycerol band containing the FAs in position
sn-2 in TAG molecules and the total FA composition in
TAG molecules are used with a computer program, LIPASE,
to calculate the original TAG composition (http://
niobio.grasa.csic.es/emforce/GGBLS/). Nevertheless this
method is suitable only to get a reference of the TAGs that
should be found in any oil sample, because of the asymmetrical
distribution of some FAs in the sn-1 and sn-3
positions of the TAGs