Fifty microliters of gliadin fraction were loaded per well. Gels
were run at 50 mA for five hours, fixed and stained with 0.23% (w/v) Coomassie Blue R-250 dissolved in 3.9% (w/v) trichloroacetic acid (TCA), 6% (v/v) acetic acid and 17% (v/v) methanol for 45 min. Destaining was performed with 8% acetic acid and 18% (v/v) ethanol