An aerobic bacterial strain P11-2 with high amylolytic activity was isolated from soil sample collected
from wheat field of Jiyuan, China. The strain was identified as Bacillus methylotrophicus by morphological
and physiological characteristics as well as by analysis of the gene encoding the 16S rRNA. The -amylase
was purified to homogeneity by a combination of 80% (NH4)2SO4 precipitation, DEAE FF anion exchange,
and superdex 75 10/300 GL gel filtration chromatography.
strain.