The overall de novo assembly was performed using the combined reads from the two libraries, generating a total of 14538 unigenes. The length distribution of these unigenes is shown in Table 2. The lengths of the unigenes range from 100 to 8750 bp, with the average length of 574 bp, a greater number than that obtained by Illumina sequencing in L. vannamei . The relatively long read lengths allow for greater statistical confidence in the de novo assembly