4. Conclusions
Among the strains developed in this work, L76M2 consistently gives improved results (concerning fermentation kinetics or
completeness, glycerol production, or tasting) over the original strain for the fermentation of grape must with high sugar content and low nitrogen availability. Increased volatile acidity seems to be compensated by glycerol production or other metabolites, since it was always preferred in wine tasting. In conclusion, adaptive laboratory evolution seems to be a
feasible way to improve the technological properties of industrial wine yeast strains, even for features that would be expected to be extremely selected for during the history of fermented foods. In order to obtain the better results, further researchwould be required concerning specific growth conditions and media composition that would guide the evolution towards the most interesting outcomes