Bacterial strains isolated from orchids able to solubilize phosphate in vitro were selected by culturing in petri dishes containing media through the following methode: Pikovskaya agar, NBRIY agar and NBRIP agar.
After 14 days of incubation at 28 c, colony diameters and the diameters of phosphate solubilization were measured.
solubilization halo size was calculated by subtracting the colony diameter from the total halo solubilization dismeter.