regeneration [25,26]. The aim of this work was to develop a novel composite hydrogel for bone tissue engineering of silk fibroin and nanoHA, an approach that has been poorly explored. The hydrogels were prepared by a new and innovative method, which can be used to form hydrogels in few minutes, using ethanol as gelling agent. The focus of the present study was to accelerate the formation of hydrogels without occurrence of nanoHA aggregation or sedimentation. The attributes of SF in combination with the features of nanoHA will form a material with interesting properties, combining the mechanical integrity and slow degradation of SF with the bioactivity and osteo- conductivity of the nanoHA.