Folin-Ciocalteu method
The extracts were investigated for their phenolic content using the Folin-Ciocalteu (FC) method [22]. Using 12 well Nunc plates, 100 μg (in 100 μL amounts) of the test solutions were mixed with FC reagent and then after mixing,20% sodium carbonate was added (total volume 3.3mL). After incubation for 2 hours measurements were recorded at 760 nm in the Cary 50 Microplate Reader.Extracts were measured using a gallic acid standard curve and equivalents were read off the straight line generated by linear regression. Trolox equivalent anti-oxidant assay The anti-oxidant capacity of the plants was measured using the ABTS+ (2,2'-azinobis (3-ethylbenzothiazoline-6-