8.3. Immuno-PCR
Immuno-PCR potentially offers a sensitive and specific method for
detecting the antigen, in which a specific DNA molecule is used as a
marker (Fig. 11). It combines the specificity of an ELISA with the
sensitivity of the assay using PCR (Sano et al., 1992). An immuno-PCR
assay has been reported for the detection of Cry proteins expressing
GM crops such as Cry1Ac (Allen et al., 2006; Zhang and Guo, 2011).