sites of cyt b (Fig. 2). Each eight juvenile crabs of E. sinensis and
E. japonica were tested for amplification for the cyt b gene to produce
a 498 bp fragment. It was found that the E. sinensis-specific
PCR method showed specificity for E. sinensis at annealing temperature
67 C (Fig. 6). Then, we conducted PCR to identify 32 juvenile
groups to be E. sinensis and E. japonica, respectively. Finally,
we confirmed that Species-specific PCR fragment from the
E. sinensis obtained by electrophoresis, which was in agreement
with the expected PCR size, and confirmed with DNA sequencing,
respectively. Based on these results, we made an assumption that
the site was single-nucleotide polymorphism (SNP) site, which
could be distinguish E. sinensis from E. japonica.