In order to investigate the demelanizing activity of G. lucidum
extract and compounds as an important factor in fungal virulence,
eight microfungi were used. Demelanizing activity was obtained
only for G. lucidum extract toward A. niger.
The results were expressed as minimum demelanizing concentrations
(MDCs), which were defined as sublethal and subinhibitory
concentration necessary to provoke demelanization in
fungus during 72 h. The subinhibitory concentration was achieved
at 0.75 mg/mL, while sublethal concentration was observed at
0.1 mg/mL of G. lucidum extract (Fig. 2a–f). The colored conidiophores
of some Aspergillus and Penicillium species contains
pigments belonging to the group of melanins: a green colored
chromoprotein and a black insoluble pigment