The column temperature was held at 30 °C for both columns.
The mobile phase components were acetonitrile:water:acetic acid
(200:800:0.5 v/v/v) at a flow rate of 1 mL/min for flavonoid determination,
and 37% solution of acetonitrile in water at a flow rate of 1.5 mL/
min for limonin. The injection volume was 20 μL