The column was held isothermally for 8 min. at 75 °C and then programmed up to 200 °C at 4°C/min. and finally held at 200°C for 4 min. The sample volume was 0.5 µl that was split at 100:1 ratio allowing about 5 µg to be placed on the column. It is seen from figure 5 that a very good separation is obtained that convincingly confirms the complex nature of the essential oil. In practice, however, the net flavor or odor impact can often be achieved by a relatively simple mixture of synthetic compounds.