The “SEPHADEX Filter Test” is an objective essay
for more than one of the properties of frozen spermatozoa.
Leakage of intracellular enzymes, in particular, glutamic
oxaloacetic transaminase (GOT) is used to assay for
membrane damage during preservation of semen (Graham
et al., 1976). More recently, the amplified restriction
fragment length polymorphism (AFLP) techniques that is
used to asses semen motility, plasma membrane integrity
and acrosome integrity in post thaw semen have been
resorted (Thurston et al.,2001)