The gene encoding a putative lipase/esterase (lp_3562) in L. plantarum
WCFS1 was amplified by PCR by using the primers 575 (50-GGTGAAAACCTGTATTTCCAGGGCatggagatacgcacactagctttaa) and 576 (50-ATCGATAAGCTTAGTTAGCTATTACTAaatttcagtactattcttgcta) (the nucleotides pairing the expression vector sequence are indicated in italics, and the nucleotides pairing the lp_3562 gene sequence are written in lowercase letters).