the use of an internal standard that can easily be prepared
chemically significantly reduces analytical errors. By using
4-(bromomethyl)-7-methoxycoumarin as a derivatizing agent for
aromatic and heterocyclic acids, isosteviol and dihydroisosteviol
can be measured by a highly specific, fluorometric detection which
is significantly more sensitive than detection methods based on UV
(Minne et al., 2004). Detection by MS on the other hand is reportedly
either more sensitive