Material and methods
Reagents
CIT 98.7% (containing 47.6% and 49.1% of NAL and GAL,
respectively), GOL 99%, NOL 99% and ergosterol were
purchased from Sigma-Aldrich® (Brazil). Lemongrass oil
from Cymbopogon citratus was obtained from Ferquima (São
Paulo, Brasil). Ecalta® (anidulafungin - AFG) was purchased
from Pfizer® (USA) and each 1 g of injectable product
contains 100 mg of AFG. Terbinafine hydrochloride (TBF)
100% and amphotericin B (AMP) 972 μg.mg-1 were kindly
supplied by Cristália® (Brazil). Methanol (Tedia®, HPLC
grade) and water filtered through a Milli-Q purification
system (Millipore®) were used for HPLC mobile phase
preparation. RPMI 1640 medium containing L-glutamine,
without sodium bicarbonate, Sigma-Aldrich® (USA), and
buffered to pH 7.0 with MOPS buffer (Sigma®).