A total of 12 SPF L. vannamei samples including adult shrimp and larvae were randomly collected from prime division of Tamil Nadu aquaculture environment for the study. The shrimps used in culture were imported from Brazil’s aquaculture grounds through CP Aqua India Pvt,Ltd and other leading aquaculture industries. The samples collected were kept in icebox and taken to lab. Collected samples were stored at -20 曟 for further use. DNA was isolated from gills, appendages, and pleopod legs, etc., from adult shrimp and larvae.