Crude papaya latex, called “papain” was used for the firsttime in 1911 for beer chill-proofing, representing the firstindustrial application of enzymes. Nowadays, papain is usedin brewing of beer in mashing process to allow high lev-els of barley versus malt, as well as in chill-proofing of clearbeer to prevent the formation of a chill-haze on cold stor-age. Moreover, since papain has an broad substrate specificity,hydrolyzing amides, esters and proteins, this protease couldfind useful application in winemaking. In a recent study,bromelain from pineapple stem, another cysteine protease,has been characterized under wine-like conditions in pres-ence of potential inhibitors naturally present in wine, showingthat this protease might find productive biotechnologicalapplication in winemaking (Benucci et al., 2011; Esti et al.,2011).