Total phenolics contents of diluted extracts (1:10) were determined
according to Alves et al. (2010). Briefly, 500 L of each
extract were mixed with 2.5 mL of the Folin–Ciocalteu reagent
(1:10) and 2 mL of a sodium carbonate solution (7.5% m/v). The mixture
was first incubated at 45 ◦C, during 15 min, followed by 30 min
incubation at room temperature before absorbance readings at
765 nm were performed. Total phenolics content was calculated
from a calibration curve prepared with gallic acid (10–100 mg/L;
r = 0.9997) and expressed as mg of gallic acid equivalents (GAE)/L
of extract.