Antifungal activity of the crude plant extract was analyzed using agar tube dilution method. Test organisms included Alternaria alternata, Aspergillus niger Fusarium oxysporum, Trichoderma harizanum, Aspergillus flavus, Aspergillus parasitica and Penecillium. Stock solution at a concentration of 24 mg/ml was prepared using sterile DMSO.
Sterile Sabouraud dextrose agar was prepared for nourishment of fungal strains. 5 ml of sterile medium and 67.6 μl stock solutions were mixed in sterile test tubes in laminar flow hood. Test tubes were placed in slanting position and cooled. After preparation of slants,fresh fungal cultures were inoculated to test tubes using flame sterilized inoculating loop.
Standard drug Miconazole and DMSO were used as positive and negative control respectively.The samples were incubated for a week at 28oC. After incubation, visible non-mycelia linear growth of fungal strains were calculated and compared.