The objectives of the study were to investigate the effect of fortification with ground Chenopodium quinoa
leaves (QL) on the sensory value and nutraceutical potential of breads and to compare an in vitro
physiological and chemical procedure for determination their quality. By QL addition, breads were
mainly enriched with rutin and gallic acid. The highest content of rutin was found in buffer extracts (from
73.55 mg/g DM to 209.89 mg/g DM depending from QL addition), whereas the highest gallic acid content
was observed after simulated digestion (from 1527.81 mg/g DM (control bread) to 2214.60 (bread with
5 g/100 g QL addition)). Antioxidant potential of enriched breads was significantly higher than activity of
control. Breads were especially fortificated with bioaccessible lipids preventers and reductive
compounds. Essential differences between both used procedures were illustrated by extractability and
fortification factors. Chemical extraction overestimates the ability to chelate metal ions, while
underestimates the ability to lipids prevention and reducing power of potentially bioaccessible and
mastication-extractable compounds. The sensory evaluation, antioxidant tests, extractability and
fortification factors showed that the replacements of wheat flour with up to 3 g/100 g QL powder in
breads gives satisfactory results. Additionally, we propose the universal protocol for preliminary
evaluation of efficiency of fortificated food.