Mortality of the fish immunized with Poly(I:C) at 17 ◦C by immersion route was 4.2% (RPS 95.8%), which is sufficient for protection of the immunized fish from VHSV-injection.
This suggests that Poly(I:C) immunization of Japanese flounder with live VHSV by immersion route also is sufficient, as demonstrated in sevenband grouper with NNV [30].