Materials and methods
2.1. Rice bran
Colored rice bran from six rice samples collected from the
local markets were obtained by polishing the colored rice
using a laboratory rice mill (VP-31T, Yamamoto Co. Ltd.,
Tendu, Japan) with the setting of 5 for flow. Four of the six
colored rice, including two black and two red rice, were
domestically grown. Two colored rice grown in Eastern
Taiwan, Taibalang black waxy rice (HB) and Taibalang red
waxy rice (HR), were purchased from Hualien KuangFeng
Farmer's Association (Hualien, Taiwan) and another one
grown in Eastern Taiwan, Guangfu red rice (GR), was
purchased from a local farmer (Hualien, Taiwan). A black
rice (WB) cultivated in western Taiwan was provided by
Yeedon Enterprise Co. Ltd. (Taipei, Taiwan). Two imported
rice samples were from Thailand, one black rice (TB) and
one red rice (TR), and provided by Yeedon Enterprise Co.
Ltd. Rice bran collected from the first pass and from the
second to forth passes of the polishing process were
defined as RB-1st (outer bran) and RB-2nd (inner bran),
respectively. The bran samples were stored at 4C until
analyses.
2.2. Proximate composition analysis