Preparation of corn-stover hydrolysate and fermentation
in shake flasks
Corn stover (CS) collected from a local farm (Henan,
China) was used as the raw material, and was dried and
milled to a small size (1 to 4 mm). Dilute sulfuric-acid
pretreatment and enzymatic hydrolysis were carried out
following the method of Wang et al. [16]. The hydrolysate
was concentrated, given an excess of lime with calcium
hydroxide and filtered. The final hydrolysate
contained 60 g/L total sugar, and was used for fermentation
without any nutrient supplementation. The
experiments were conducted at 30°C with pH 6.0.