Fig. A The result show that at 30 °C host strain grow better than mutants and at 42 °C four mutant grew better than host strain at 48 and 72 h.
And fig. B when five strain were spread on YPD and GBA medium The result show that all strains can grow on YPD medium but mutant cannot grow on GBA. Indicating that GBA is selective medium for respiration-deficient mutant.