from 2.5 to 2.6 (mean 2.5) and 0.406 to 0.410 (mean 0.407) respectively in lake populations (Table 1) While most loci were in HWE 24 out of 115 cases showed significant deviations from HWE expectations including 13 for pond populations and 11 for lake populations (Table S1 , Supporting information) The analysis of LD between pairs of microsatellite loci revealed a much lower number of loci in LD for pond than for lake populations While ponds had 0.5% of locus pairs in LD lakes had 2.9-12.2% (mean:8.8%) of locus pairs in LD