at pH 2.0 (▫), 3.0 (-), 8.0 (:), and at pH 2.0 in presence of wholemeal wheat bread (); further intestinal digestion (SIJ; 180e360 min) was carried out at pH 8.0. The values are the average of three replicates and the standard deviations were indicated by vertical bars.
3.7. Hydrolysis of phytate by S. cerevisiae LC3 under simulated GI conditions
The hypothesis to be investigated concerned the possibility to use S. cerevisiae LC3 as probiotic to hydrolyse phytate during GI digestion. Phytate was hydrolysed under GI digestion by LC3 and no phytate was found after incubation for 24 h at 37 C (Fig. 5). Previously, Haraldsson et al. (2005) showed that high-phytase S. cerevisiae mutant strains BY80 and BY85 survived and hydrolysed phytate under simulated GI digestion. Unlike Haraldsson et al. (2005), under our experimental conditions, at the end of the simulated GI digestion, ca. the 60% of phytate was hydrolysed and no phytate was found after incubation for 24 h at 37 C.