The objectives of the current study were, firstly to determine the molecular weight ofcondensed tannins (CTs) of 62-2-8 Leucaena-hybrid Bahru (LLB) and relating it to its proteinbinding ability, using the local Leucaena leucocephala (LLL) as control and; secondlyto evaluate the effect of different levels of CTs of the hybrid LLB on rumen fermentationparameters, including dry matter and nitrogen digestibility and methane gas production.The weight-average molecular weights (Mw) of purified CTs extracted from hybrid LLB andlocal LLL, determined using gel permeation chromatography were near similar, being 2737and 2872 Da, respectively. However, the CTs of the hybrid LLB exhibited stronger proteinbinding ability than the local LLL (0.305 and 0.420 mg, respectively, for LLB and LLL to bindhalf maximum bovine serum albumin). The above results seems to suggest that molecularmass of CTs, at least for Leucaena forages, is not the sole factor in determining theircapability to bind protein. Inclusion of about 20–40mg of CT/g DM of the CTs from hybridLLB effectively decreased (P<0.05) methane production and in vitro nitrogen digestibility.The above results suggested a potential of using CTs from the hybrid LLB to protect dietaryprotein from rumen degradation for better utilization in the small intestine of ruminantanimals, and in addition to mitigate their enteric methane emission, often implicated as aแหล่งที่มาของก๊าซเรือนกระจก
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