Helium was the
carriergas,flowratewas25 mL/min.Ovenanddetectortemperature was 300 C. Total reducing sugars ware determined by the3,5-dinitro salicylic acid (DNS) method [18] and glucose was
used as a standard. The samples were stored in a fridge at
18 C until analysis to prevent spoilage by microbes and loss
of ethanol.