epithelial origin
isolated
confirmed
subjecting
immunocytochemical
staining
expression
cytokeratins
using
monoclonal
mouse
antihuman
cytokeratin
antibodies
biotinylated
goat
antimouse
antibodies
streptavidin–horseradish peroxidase
substrate-chromogen
according
manufacturer
instructions.
Bovine
oviductal
four-well culture plates
as freshly retrieved BOECs
as first-passage BOECs
culture period
culture flask
increase cell
purity
trypsinized,
reseeded
glass inlets
freshly retrieved BOECs
cultured
monolayers
hanging
inserts