Particularly, in this application the term sugar yield refers to the yield of water soluble เปเปโอลิโกเมอร์, เปเปไดเมอร์ and เปเปโมโนเมอร์ carbohydrates derived from
25 lignocellulosic materials.
Single cell oil production process
"Single cell oil production process" refers here to a process, ซึ่งประกอบรวมด้วย steps of forming or allowing the growth of a ไลปิด synthesizing microorganism and allowing the thus obtained organism mass to produce and/or store (accumulate) ไลปิด,
30 recovering the cells from the liquid phase, and extracting or recovering the ไลปิด from the cells. In certain cases, single cell oil can be also extracellular such as excreted or liberated from cells in culture medium during or after cultivation.
Aerobic cultivation
The term "aerobic cultivation" or "aerobic fermentation" refers to a cultivation where the microorganism utilizes oxygen as terminal electron acceptor for energy
5 generation (i.e. microorganism uses aerobic respiration). ตามปกติ in เครื่องปฏิกรณ์ชีวภาพ, aerobic cultivation is performed by adding oxygen or a gas ของผสม containing oxygen (ตามปกติ air), i.e. เครื่องปฏิกรณ์ชีวภาพ is aerated. When microorganisms uses aerobic respiration in cultivation, it can be referred as "cultivation under aerobic conditions".
ตามปกติ this occurs in aerated เครื่องปฏิกรณ์ชีวภาพ.
10
Aseptic operation
The term "aseptic operation" refers here operation where microorganism cultivation systems (e.g. fermenter) have been sterilized prior to cultivation, and where operation is performed in a way which prevents contamination (i.e. growth of non-
15 desired microorganisms) of cultivation systems, e.g. by using antimicrobial agents not derived from lignocellulose pre-treatment. "Non-aseptic operation" refers operation performed otherwise than "aseptic operation"
จุลินทรีย์สะสมน้ำมัน or Oil producing microorganism
20 The จุลินทรีย์สะสมน้ำมัน (also refer to as oil producing organisms) used in the การประดิษฐ์นี้ are selected from the group of bacteria, cyanobacteria, fungi such as yeasts and filamentous fungi, archaea or microalgae. The microorganisms can readily accumulate ไลปิด or have been genetically modified to accumulate ไลปิด or
to improve accumulation of ไลปิด.
25
ที่ควรใช้คือ organisms that are capable of utilizing C6 and C5 sugars are used. ที่ควรใช้คือ organisms are yeast, filamentous fungi or bacteria.
In the context of the การประดิษฐ์นี้, the จุลินทรีย์สะสมน้ำมัน (จุลินชีพสะสมน้ำมัน) refers to a microorganism which is capable of accumulating intercellular ไลปิด such that the ไลปิด mounts at least 15 % (w/w) of the total biomass (per cell dry weight) of the microbe when it is cultivated under suitable conditions. In a
preferred embodiment, the จุลินทรีย์สะสมน้ำมัน is capable of accumulating at least
20 % (w/w) of the total biomass of the microbe (per cell dry weight).
Preferred microorganism strains for the purposes of the การประดิษฐ์นี้ include,
5 but are not limited to, the species and genera listed below:
According to one embodiment of the การประดิษฐ์, the first microbe is an จุลินทรีย์สะสมน้ำมัน capable of utilizing sugars derived from lignocellulosic materials. ที่ควรใช้คือ, จุลินทรีย์สะสมน้ำมัน are capable of utilizing C6 sugars (six carbon sugars, such as
10 glucose, mannose and galactose) and C5 sugars (such as xylose and arabinose) in ไฮโดรไลเซตประเภทลิกโนเซลลูโลสs. According to one embodiment of the การประดิษฐ์, the จุลินทรีย์สะสมน้ำมัน is capable of utilizing polymeric or เปเปโอลิโกเมอร์ carbohydrates in lignocellulose or fractions thereof.
15 Preferred (filamentous) fungal strains are from species from genera Aspergillus such as