Samplesofdeep-oceanwater(3170m)take

Samplesofdeep-oceanwater(3170m)takenfromtheRockallTrough(North-EastAtlantic)were
incubatedforone-monthatatmosphericand in-situ pressure(31MPa),at4 1C andintheabsenceand
presence ofaddednutrients.Prokaryoticabundance(directcellcounts)increasedbyatleast28-foldin
enrichmentswithoutaddednutrients.However,themagnitudeofincreaseinabundancewaslessfor
incubationscarriedoutat in-situ pressure(131–181-fold)thanthoseincubationsatsurfacepressure
(163–1714-foldincreaseinabundance).Changesintheprokaryoticcommunityprofileasaresultof
one-monthincubationweremeasuredbymeansofDenaturingGelElectrophoresis(DGGE)ofextracted
16S rDNA.Theprofilesofpost-incubationsamplesincubatedat in-situ pressurewereseparatedfromall
other profilesaswerethoseofunpressurisedsampleswithaddednutrients.Thebehaviour(fitness)of
individualcommunitymembers(OperationalTaxonomicUnits:OTUs)wasdeterminedonthebasisof
changeinrelativeDGGEbandintensitiesbetweenpre-andpost-incubationsamples.Oftwenty-one
OTUs examined,sixwerefitterwhenincubatedinthepresenceofaddednutrientsandat in-situ
pressureandoneofthesewasadvantagedwhengrownintheabsenceofaddednutrientsandat in-situ
pressure.Theserepresentedautochthonousandactivemembersofthedeep-oceanprokaryotic
community.Incontrast,sevenOTUsweredisadvantagedwhengrownunder in-situ pressureandwere
indicativesurface-derivedallochtonousmicroorganisms.AfurthertwoOTUscametodominancein
incubationswithaddednutrients(pressurisedandunpressurised)andsimilartothepreviouscategory
were probablysurface-derivedmicroorganisms.AsingleOTUshowedcharacteristicsofpiezophilicand
oliogrophicbehaviourandfourOTUsweredisadvantagedunderallincubationconditionsexamined.
The twenty-oneDGGEbandsweresequencedandthebacterialcommunitiesweredominatedby
Gammaproteobactria and toalesserextentmembersofthe Cytophaga–Flavobacterium–Bacteroides and
d groups ofprokaryotes.

ผลลัพธ์ (ไทย) 1:

Samplesofdeep oceanwater(3170m) takenfromtheRockallTrough(North-EastAtlantic) ได้ความดันในซิ incubatedforone monthatatmosphericand (31MPa), at4 1C andintheabsenceandofaddednutrients อยู่Prokaryoticabundance (directcellcounts) increasedbyatleast28-foldinenrichmentswithoutaddednutrientsอย่างไรก็ตาม themagnitudeofincreaseinabundancewaslessforthanthoseincubationsatsurfacepressure incubationscarriedoutat ในการวิเคราะห์ความดัน (131-181-พับ)(163-1714-foldincreaseinabundance)Changesintheprokaryoticcommunityprofileasaresultofofextracted หนึ่ง-monthincubationweremeasuredbymeansofDenaturingGelElectrophoresis (DGGE)Pressurewereseparatedfromall ในซิ rDNA.Theprofilesofpost incubationsamplesincubatedat 16Sprofilesaswerethoseofunpressurisedsampleswithaddednutrients อื่น ๆThebehaviour (ฟิตเนส) ของindividualcommunitymembers(OperationalTaxonomicUnits:OTUs) wasdeterminedonthebasisofchangeinrelativeDGGEbandintensitiesbetweenpre-andpost-incubationsamples.Oftwenty-1ตรวจสอบ OTUs sixwerefitterwhenincubatedinthepresenceofaddednutrientsandat ในซิpressureandoneofthesewasadvantagedwhengrownintheabsenceofaddednutrientsandat ในซิความกดดันTheserepresentedautochthonousandactivemembersofthedeep-oceanprokaryoticชุมชนIncontrast, sevenOTUsweredisadvantagedwhengrownunder ในซิ pressureandwereindicativesurface-derivedallochtonousmicroorganismsAfurthertwoOTUscametodominanceinandsimilartothepreviouscategory incubationswithaddednutrients (pressurisedandunpressurised)probablysurface-derivedmicroorganisms ได้AsingleOTUshowedcharacteristicsofpiezophilicandoliogrophicbehaviourandfourOTUsweredisadvantagedunderallincubationconditionsexaminedยี่สิบ-oneDGGEbandsweresequencedandthebacterialcommunitiesweredominatedbyGammaproteobactria และ toalesserextentmembersofthe Cytophaga – Flavobacterium – Bacteroides และd กลุ่ม ofprokaryotes

ผลลัพธ์ (ไทย) 2:

ความดันในแหล่งกำเนิด (31MPa) AT4 1C andintheabsenceand
การแสดงตน ในแหล่งกำเนิด rDNA.Theprofilesofpost-incubationsamplesincubatedat ในแหล่งกำเนิด pressurewereseparatedfromall อื่น ๆ ตรวจสอบ sixwerefitterwhenincubatedinthepresenceofaddednutrientsandat ในแหล่งกำเนิดpressureandoneofthesewasadvantagedwhengrownintheabsenceofaddednutrientsandat ในแหล่งกำเนิด ยี่สิบ oneDGGEbandsweresequencedandthebacterialcommunitiesweredominatedby Gammaproteobactria และ toalesserextentmembersofthe Cytophaga-Flavobacterium-Bacteroides และกลุ่ม D ofprokaryotes

ผลลัพธ์ (ไทย) 3:

samplesofdeep oceanwater ( 3170m ) takenfromtherockalltrough ( เหนือ eastatlantic )
incubatedforone ควบคู่ monthatatmosphericand ความดัน ( 31mpa ) at4 1C andintheabsenceand
ตน ofaddednutrients . prokaryoticabundance ( directcellcounts ) increasedbyatleast28 foldin themagnitudeofincreaseinabundancewaslessfor

enrichmentswithoutaddednutrients อย่างไรก็ตามความดัน incubationscarriedoutat ควบคู่ ( 131 ) 181 พับ ) thanthoseincubationsatsurfacepressure
( 163 ) 1714 foldincreaseinabundance ) changesintheprokaryoticcommunityprofileasaresultof
1 monthincubationweremeasuredbymeansofdenaturinggelelectrophoresis ( การทดลอง ) ofextracted

( rdna.theprofilesofpost-incubationsamplesincubatedat ควบคู่ pressurewereseparatedfromallprofilesaswerethoseofunpressurisedsampleswithaddednutrients อื่น thebehaviour ( ฟิตเนส )
individualcommunitymembers ( operationaltaxonomicunits : ใน ) wasdeterminedonthebasisof
changeinrelativedggebandintensitiesbetweenpre andpost incubationsamples . oftwenty หนึ่ง

sixwerefitterwhenincubatedinthepresenceofaddednutrientsandat ควบคู่ในตรวจสอบpressureandoneofthesewasadvantagedwhengrownintheabsenceofaddednutrientsandat ควบคู่ oceanprokaryotic

ดัน ชุมชน theserepresentedautochthonousandactivemembersofthedeep . และ sevenotusweredisadvantagedwhengrownunder ควบคู่ pressureandwere derivedallochtonousmicroorganisms afurthertwootuscametodominancein

indicativesurface .incubationswithaddednutrients ( pressurisedandunpressurised ) andsimilartothepreviouscategory
ถูก probablysurface derivedmicroorganisms . asingleotushowedcharacteristicsofpiezophilicand


20 onedggebandsweresequencedandthebacterialcommunitiesweredominatedby oliogrophicbehaviourandfourotusweredisadvantagedunderallincubationconditionsexamined .และ gammaproteobactria toalesserextentmembersofthe cytophaga –ฟลาโวแบคทีเรียม ( bacteroides
D และกลุ่ม ofprokaryotes .