Following to the method of Kreger-van Rij (1984) and Kurtzman and Fell
(1997), ascospore formation is use for indication of the ascomycetous yeasts. As
shown in Figure 4.6, ascospore formed on YPD agar after 3 weeks at 25 °C. Asci are
persistent and form 1-2 spheroidal ascospores.