Discussion
Cross-species transferability and polymorphisms of cucumber SSRs in the melon genome
Nearly 2,400 randomly sampled cucumber genomic SSRs were tested herein for their ability to detect polymorphisms among three melon parental lines used in map construction. The polymorphism level of cross-species transferable cucumber SSRs was 11.1% between
Top Mark and WI 846-1, and 16.9% between Top Mark and line Q3-2-2, which would be reduced to 5.9 and 7.7%, respectively if all cucumber SSRs tested were considered.
This level was appreciably lower than that reported in other studies [35,37] using melon SSRs. For example, of 492 melon SSRs evaluated for map construction by Cuevas et al. [35], 32% were polymorphic between two melon lines Q3-2-2 and Top Mark.Likewise, the level of polymorphism of melon SSRs between the two melon lines PI 414723 and Dulce used
by Harel-Beja et al. [37] was comparatively high (as much as 55%).
Discussion
Cross-species transferability and polymorphisms of cucumber SSRs in the melon genome
Nearly 2,400 randomly sampled cucumber genomic SSRs were tested herein for their ability to detect polymorphisms among three melon parental lines used in map construction. The polymorphism level of cross-species transferable cucumber SSRs was 11.1% between
Top Mark and WI 846-1, and 16.9% between Top Mark and line Q3-2-2, which would be reduced to 5.9 and 7.7%, respectively if all cucumber SSRs tested were considered.
This level was appreciably lower than that reported in other studies [35,37] using melon SSRs. For example, of 492 melon SSRs evaluated for map construction by Cuevas et al. [35], 32% were polymorphic between two melon lines Q3-2-2 and Top Mark.Likewise, the level of polymorphism of melon SSRs between the two melon lines PI 414723 and Dulce used
by Harel-Beja et al. [37] was comparatively high (as much as 55%).
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