In the first phase, the accumulated black and glutinous organic sludge was layered onto the pond soils and then studied on the effectiveness of various pond bottom soil containing organic sludge treatments on changes in pH, organic matter, total heterotrophic bacteria, Vibrio and Pseudomonas. The initial pH of the control and treated groups, 7.19 ± 0.17 to 7.41 ± 0.14, did not differ significantly (P > 0.05; Table 1). In control tanks, pH of pond soils at the beginning of experiment was 7.19 ± 0.17, and decreased to 6.77 ± 0.14 (P < 0.05) on day 3. Soils dried in sunlight and tilled (treatment 1) also had a significantly lower pH of 7.19 ± 0.07 (P < 0.05) at day 3, followed by a further decline to 6.62 ± 0.15 (P < 0.05) at the end of experiment (day 15). Similarly, soil dried in sunlight with the addition of probiotic (treatment 4) or dried in sunlight and tilled along with the addition of probiotics (treatment 5) declined in pH (P < 0.05) by day 3. In contrast, soil treated with sunlight, tilling and lime addition (treatment 2) or sunlight and lime addition (treatment 3), pH declined significantly (P < 0.05) only after 9 days.
In the first phase, the accumulated black and glutinous organic sludge was layered onto the pond soils and then studied on the effectiveness of various pond bottom soil containing organic sludge treatments on changes in pH, organic matter, total heterotrophic bacteria, Vibrio and Pseudomonas. The initial pH of the control and treated groups, 7.19 ± 0.17 to 7.41 ± 0.14, did not differ significantly (P > 0.05; Table 1). In control tanks, pH of pond soils at the beginning of experiment was 7.19 ± 0.17, and decreased to 6.77 ± 0.14 (P < 0.05) on day 3. Soils dried in sunlight and tilled (treatment 1) also had a significantly lower pH of 7.19 ± 0.07 (P < 0.05) at day 3, followed by a further decline to 6.62 ± 0.15 (P < 0.05) at the end of experiment (day 15). Similarly, soil dried in sunlight with the addition of probiotic (treatment 4) or dried in sunlight and tilled along with the addition of probiotics (treatment 5) declined in pH (P < 0.05) by day 3. In contrast, soil treated with sunlight, tilling and lime addition (treatment 2) or sunlight and lime addition (treatment 3), pH declined significantly (P < 0.05) only after 9 days.
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