Spectra photometrical experiment
We have used this method only for one sample as Chromium is a highly carcinogenic material and the experiment with this chemical reagent is very hazardous and In this method we have to use a chemical reagent named pyridinium chlorochromate(C5H5N. Clcro3H). In this process some diluted standard solution like 0.1 0.2.7 0.8%.....1% is made with standard ethanol and distilled water. After that 5 ml of our very prepared standard solution is mixed with 2 ml of pyridinium chlorochromate solution. It is then kept in complete darkness for 1hr. Then using the spectra photometer absorbance at 588 nm concentration of different solution is achieved. Then our ethanol solution and pyridinium chlorochromate solution is used to get the absorbance. Then comparing with the standard data we got the % ethanol present in the solution.