Biosurfactants are heterogeneous group of surface active
chemical compounds produced by a wide variety of microorganisms
[57, 58, 60, 81–83]. Surfactants enhance solubilization
and removal of contaminants [84, 85]. Biodegradation
is also enhanced by surfactants due to increased bioavailability
of pollutants [86]. Bioremediation of oil sludge
using biosurfactants has been reported by Cameotra and
Singh [87]. Microbial consortium consisting of two isolates
of Pseudomonas aeruginosa and one isolate Rhodococcus
erythropolis from soil contaminated with oily sludge was
used in this study. The consortium was able to degrade
90% of hydrocarbons in 6 weeks in liquid culture. The
ability of the consortium to degrade sludge hydrocarbons
was tested in two separate field trials. In addition, the
effect of two additives (a nutrient mixture and a crude
biosurfactant preparation on the efficiency of the process
was also assessed. The biosurfactant used was produced by a
consortium member and was identified as being a mixture of
11 rhamnolipid congeners. The consortium degraded 91% of
the hydrocarbon content of soil contaminated with 1% (v/v)
crude oil sludge in 5 weeks. Separate use of any one additive
along with the consortium brought about a 91–95% depletion
of the hydrocarbon content in 4 weeks, with the crude
biosurfactant preparation being a more effective enhancer
of degradation. However, more than 98% hydrocarbon
depletion was obtained when both additives were added
together with the consortium. The data substantiated the use
of a crude biosurfactant for hydrocarbon remediation.
Biosurfactants are heterogeneous group of surface activechemical compounds produced by a wide variety of microorganisms[57, 58, 60, 81–83]. Surfactants enhance solubilizationand removal of contaminants [84, 85]. Biodegradationis also enhanced by surfactants due to increased bioavailabilityof pollutants [86]. Bioremediation of oil sludgeusing biosurfactants has been reported by Cameotra andSingh [87]. Microbial consortium consisting of two isolatesof Pseudomonas aeruginosa and one isolate Rhodococcuserythropolis from soil contaminated with oily sludge wasused in this study. The consortium was able to degrade90% of hydrocarbons in 6 weeks in liquid culture. Theability of the consortium to degrade sludge hydrocarbonswas tested in two separate field trials. In addition, theeffect of two additives (a nutrient mixture and a crudebiosurfactant preparation on the efficiency of the processwas also assessed. The biosurfactant used was produced by aconsortium member and was identified as being a mixture of11 rhamnolipid congeners. The consortium degraded 91% ofthe hydrocarbon content of soil contaminated with 1% (v/v)crude oil sludge in 5 weeks. Separate use of any one additivealong with the consortium brought about a 91–95% depletionof the hydrocarbon content in 4 weeks, with the crudebiosurfactant preparation being a more effective enhancerof degradation. However, more than 98% hydrocarbonการลดลงของได้รับเมื่อมีเพิ่มสารทั้งสองร่วมกับกิจการร่วมค้า ข้อมูล substantiated ใช้ของ biosurfactant ที่ดิบสำหรับไฮโดรคาร์บอนเพื่อ
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