Additionally, we used commercial HCT medium as the reference medium (M4), which contained 5 gL-1 Bacto Tryptone (Difco) and 2 gL-1 Casamino acids (Difco); the pH was adjusted to 7.2, and after sterilization, 3.4 gL-1 KH2PO4, 0.012
gL-1 MgSO4.7H2O. 0.003 gL-1 MnSO4.4H2O, 0.0028 gL-1 ZnSO4.7H2O, 0.02 gL-1 Fe (SO4)3.7H2O, 0.147 gL-1 CaCl2.2H2O, and 3 gL-1 glucose were added