FS13 employed for the FLOTAC analysis performed slightly better
than FS4 for diagnosing Trichuris spp. eggs, revealing four out
of seven cases with large eggs before treatment (FS4: 5/7; Kappa:
0.42, indicating moderate agreement) and 17 of 22 cases after treatment
(FS4: 13/22; Kappa: 0.47, moderate agreement). Using FS13,
all but one case of T. trichiura infection with standard-sized eggs
were detected (Kappa before and after treatment: 0.79 and 0.78,
both indicating substantial agreement; data not shown).