The soil samples were collected from various
locations in and around Belgaum, Karnataka,
India, dried separately at 45 °C for 1 h in a
hot air oven and then cooled to room
temperature. The soil sample (1 g) was
added to a conical flask containing 100 ml of
sterile water and few drops of Tween 80. The
flasks were shaken for 30 min in an orbital
shaker incubator at 27 °C and their contents
designated stock cultures