In summary, the salt-sensitive nature of transgenic nhaA was
determined in transgenic rice callus and rice plants. Overexpression
of the nhaA gene resulted in a high death rate in transgenic materials.
This research suggests that the plasma membrane may only be
targeted by the nhaA gene when attempting to increase rice salt
tolerance. Alternatively, careful design of experiments with the
same promoter and same assay method are still required to validate
previous findings prior to large scale developmental efforts for
commercialization.