A Gram-negative, non-sporulating, non-flagellated rod, designated BR-9T, was isolated from soil
collected on the Korean peninsula. Strain BR-9T grew optimally at pH 6.0–7.0, at 30 6C and in
the absence of NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that
strain BR-9T belonged to the genus Pedobacter and clustered with Pedobacter insulae DS-139T
and Pedobacter koreensis WPCB189T. Strain BR-9T exhibited 98.2 and 97.5% 16S rRNA gene
sequence similarity with P. insulae DS-139T and P. koreensis WPCB189T, respectively, and
,96.7% sequence similarity with the type strains of other species in the genus Pedobacter.
Strain BR-9T contained MK-7 as the predominant menaquinone and iso-C15 : 0 and summed
feature 3 (C16 : 1v7c and/or iso-C15 : 0 2-OH) as the major fatty acids. The DNA G+C content of
strain BR-9T was 38.5 mol%. DNA–DNA relatedness between strain BR-9T and P. insulae
DS-139T and P. koreensis KCTC 12536T was 3.4–4.2 %, which indicated that the isolate was
genetically distinct from these type strains. Strain BR-9T was also distinguishable by differences
in phenotypic properties. On the basis of the data presented, strain BR-9T is considered to
represent a novel species of the genus Pedobacter, for which the name Pedobacter boryungensis
sp. nov. is proposed. The type strain is BR-9T (5KCTC 23344T 5CCUG 60024T).
The genus