Loofa sponge preparation
Dried fibrous loofa sponge was
organically planted and harvested for use as
supporting material with A. aceti WK. It was cut
into pieces 2.5 cm thick as mentioned in previous
investigations (Krusong et al., 2007) and shown
in Figure 1. After cutting, each piece of dried loofa
sponge was washed under running tap water and
dipped in vinegar containing 4% acetic acid for
sterilization (Krusong, 2008).