Fatty acid methyl esters were identified by comparison with retention times of 37 fatty acid methyl ester standard compound analyzed at the same conditions. With samples and standards, for every analysis, certified reference material (CRM), was prepared and analyzed at the same conditions. The result is expressed as percentage (%) individual fatty acids to total fatty acids determined. The detection limit method was 0.1%. Values determined in the validation process for parameter truthfulness were compared with the criterion of the Guidelines for the implementation of analytical methods and interpretation of results (N.N 2/2005), that to prove the truth of the proportion by weight > 10 mg/kg may vary from -20% to +10% as compared to the certified value.