2.1.1. Fungal and plant material
The AMF used were: Glomus mosseae Gerd & Trappe 1974, isolate IMA1 from UK and isolate AZ225C from USA , and Glomus intraradices N.C. Schenck & G.S. Sm. 1982, isolate IMA5 from and isolate IMA6 from France. They were obtained from pot cultures maintained in the collection of the Department of Crop Plant Biology, University of Pisa, Italy. The plant species used was the forage legume T.alexandrinum L. cv. Tigri.