Total
adiponectin concentration was measured by ELISA (Linco
Research, St. Charles, MI, USA) [33]. For determination of
fetuin-A, an immunoturbidimetric method was used with
specific polyclonal goat anti-human fetuin-A antibodies to
human fetuin-A (BioVendor Laboratory Medicine, Modreci,
Czech Republic) [34]. All assay procedures were
performed as described by the manufacturer.