Arti fi cial seawater salts (Hobby-Marin, Dohse Aquaritik AG,
Gelsdorf, Germany).
2. 172b liquid medium: Add about 800 mL distilled water to a
glass beaker. Weigh in 10 g of glucose, 20 g of soluble starch,
5 g of yeast extract, 5 g of tryptone, and 10 g of arti fi cial seawater
salts. Adjust to pH 7, and add 1 g of calcium carbonate
(see Note 1). Make up to 1 L with distilled water. Autoclave at
121°C for 20 min.
3. 172b solid medium: 172b liquid medium and 15 g of agar.
Autoclave at 121°C for 20 min.
4. ISP1 with marine salts: 5 g of tryptone, 3 g of yeast extract, and
10 g of arti fi cial seawater salts. Make up to 1 L with distilled
water. pH should be 7.0–7.2. Autoclave for 15 min at 121°C.