2.2.2. Genome information and DNA methylation analysis
The genome information was analyzed by amplified fragment length
polymorphism (AFLP) molecular markers. The experimental details
referred to the method from Hao et al. [23]. The DNA methylation was analyzed with methylation sensitive amplification polymorphism
(MSAP) molecular markers based on the method in previous study,
with minor changes [24]. (All the enzymes were purchased from
TaKaRa Company).
The adapters and primers involved in AFLP and MSAP molecular
markers were synthesized by Shanghai Sangon Company (Table 1).